Complexin 1, 2 (AA 45-81) antibody

Catalog No. ABIN1742265

Cited in 5+ publications. The Rabbit Polyclonal anti-Complexin 1, 2 antibody (ABIN1742265) specifically detects Complexin 1, 2 in ICC, IHC, IHC (p), IP and WB. The antibody is reactive with Mouse, Rat, Human, Cow, Guinea Pig, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for Complexin 1, 2 (AA 45-81) antibody (ABIN1742265)

Target: Complexin 1, 2

Reactivity: Mouse, Rat, Human, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Grade: KO Validated

Product Details

Binding Specificity: AA 45-81

Purpose: anti-Complexin1/2

Specificity: Recognizes complexin 1 and 2.

Cross-Reactivity (Details): Reacts with: human (O14810, Q6PUV4), rat (P63041, P84087), mouse (P63040, P84086), Guinea pig, cow, zebrafish, rabbit. Other species not tested yet.

Purification: antiserum

Immunogen: Synthetic peptide corresponding to AA 45 to 81 from mouse Complexin2 (UniProt Id: P84086)

Application Details

Application Notes: WB:1 : 1000 up to 1 : 20000 ICC:1 : 200 up to 1 : 500, IHC:1 : 500, IHC-P:1 : 200,

Comment:

IP: Co-immunoprecipitates the SNARE complex.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References

Getz, Visser, Bell, Xu, Flynn, Zaidi, Syed: "Two proteolytic fragments of menin coordinate the nuclear transcription and postsynaptic clustering of neurotransmitter receptors during synaptogenesis between Lymnaea neurons." in: Scientific reports, Vol. 6, pp. 31779, (2016) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Tsai, Brewis, van Maaren, Gadella: "Involvement of complexin 2 in docking, locking and unlocking of different SNARE complexes during sperm capacitation and induced acrosomal exocytosis." in: PLoS ONE, Vol. 7, Issue 3, pp. e32603, (2012) (PubMed).

Lee, Brecha: "Immunocytochemical evidence for SNARE protein-dependent transmitter release from guinea pig horizontal cells." in: The European journal of neuroscience, Vol. 31, Issue 8, pp. 1388-401, (2010) (PubMed).

Falkowski, Thomas, Groblewski: "Complexin 2 modulates vesicle-associated membrane protein (VAMP) 2-regulated zymogen granule exocytosis in pancreatic acini." in: The Journal of biological chemistry, Vol. 285, Issue 46, pp. 35558-66, (2010) (PubMed).

Target Details

Target: Complexin 1, 2

Alternative Name: Complexin 1/2

Background: CPX 1, CPX1, CPX 2, CPX2, Synaphin-1, complexin 1, complexin 2, SNARE associated, eye-retina, 2

UniProt: P84086