Rab-GDI 1 (AA 1-447) antibody

Catalog No. ABIN1742290

This Mouse Monoclonal antibody specifically detects Rab-GDI 1 in IP and WB. It exhibits reactivity toward Cow, Hamster, Human, Mouse and Rat. It has been mentioned in 17+ publications

Quick Overview for Rab-GDI 1 (AA 1-447) antibody (ABIN1742290)

Target: Rab-GDI 1

Reactivity: Cow, Hamster, Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Clone: 81-2

Product Details

Binding Specificity: AA 1-447

Purpose: Mouse anti-Rab-GDI1 IgG

Specificity: Recognizes Rab-GDI-alpha and -beta.

Cross-Reactivity (Details): Reacts with: human (P31150), rat (P50398), mouse (P50396), hamster, cow. Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 1 to 447 from bovine Rab-GDI1 (UniProt Id: P21856)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 1000 up to 1 : 10000 ICC:not tested yet IHC:not recommended IHC-P:not tested yet

Comment:

Immunoprecipitates Rab-GDI complexes.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References

Do, Chai, Casey, Wang: "Isoprenylcysteine carboxylmethyltransferase function is essential for RAB4A-mediated integrin β3 recycling, cell migration and cancer metastasis." in: Oncogene, Vol. 36, Issue 41, pp. 5757-5767, (2017) (PubMed).

Binotti, Pavlos, Riedel, Wenzel, Vorbrüggen, Schalk, Kühnel, Boyken, Erck, Martens, Chua, Jahn: "The GTPase Rab26 links synaptic vesicles to the autophagy pathway." in: eLife, Vol. 4, pp. e05597, (2015) (PubMed).

Kummer, Hammerschmidt, Martinez, Terwel, Eichele, Witten, Figura, Stoll, Schwartz, Pape, Schultze, Weinshenker, Heneka, Urban: "Ear2 deletion causes early memory and learning deficits in APP/PS1 mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 26, pp. 8845-54, (2014) (PubMed).

Chen, Wislet-Gendebien, Samuel, Visanji, Zhang, Marsilio, Langman, Fraser, Tandon: "α-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity." in: The Journal of biological chemistry, Vol. 288, Issue 11, pp. 7438-49, (2013) (PubMed).

Ahmed, Wittenmayer, Kremer, Hoeber, Kiran Akula, Urlaub, Islinger, Kirsch, Dean, Dresbach: "Mover is a homomeric phospho-protein present on synaptic vesicles." in: PLoS ONE, Vol. 8, Issue 5, pp. e63474, (2013) (PubMed).

Kaeser, Deng, Wang, Dulubova, Liu, Rizo, Südhof: "RIM proteins tether Ca2+ channels to presynaptic active zones via a direct PDZ-domain interaction." in: Cell, Vol. 144, Issue 2, pp. 282-95, (2011) (PubMed).

Burré, Sharma, Tsetsenis, Buchman, Etherton, Südhof: "Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro." in: Science (New York, N.Y.), Vol. 329, Issue 5999, pp. 1663-7, (2010) (PubMed).

Pavlos, Grønborg, Riedel, Chua, Boyken, Kloepper, Urlaub, Rizzoli, Jahn: "Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 40, pp. 13441-53, (2010) (PubMed).

Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

Sharma, Burré, Südhof: "CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity." in: Nature cell biology, Vol. 13, Issue 1, pp. 30-9, (2010) (PubMed).

Fogel, Akins, Krupp, Stagi, Stein, Biederer: "SynCAMs organize synapses through heterophilic adhesion." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 46, pp. 12516-30, (2007) (PubMed).

Schütz, Zilly, Lang, Jahn, Bruns: "A dual function for Munc-18 in exocytosis of PC12 cells." in: The European journal of neuroscience, Vol. 21, Issue 9, pp. 2419-32, (2005) (PubMed).

Huang, You, Wu, Hsu et al.: "Rap1-induced p38 mitogen-activated protein kinase activation facilitates AMPA receptor trafficking via the GDI.Rab5 complex. Potential role in (S)-3,5-dihydroxyphenylglycene-induced long term ..." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12286-92, (2004) (PubMed).

Riedel, Antonin, Fernandez-Chacon, Alvarez de Toledo, Jo, Geppert, Valentijn, Valentijn, Jamieson, Südhof, Jahn: "Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6487-97, (2002) (PubMed).

Chou, Jahn: "Binding of Rab3A to synaptic vesicles." in: The Journal of biological chemistry, Vol. 275, Issue 13, pp. 9433-40, (2000) (PubMed).

Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

Schlüter, Schnell, Verhage, Tzonopoulos, Nicoll, Janz, Malenka, Geppert, Südhof: "Rabphilin knock-out mice reveal that rabphilin is not required for rab3 function in regulating neurotransmitter release." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 14, pp. 5834-46, (1999) (PubMed).

Target Details

Target: Rab-GDI 1

Alternative Name: Rab-GDI1

Background:

Synonyms: GDI 1, Rab GDP dissociation inhibitor alpha, Rab GDI alpha, Guanosine diphosphate dissociation inhibitor 1, SMG p25A GDI

Antigen Information: The cytoplasmatic protein Rab-GDI binds to GDP-Rabs

UniProt: P21856