Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 596-705) antibody

Catalog No. ABIN1742305

This Rabbit Polyclonal antibody specifically detects not_set in WB, ICC and IHC (p). It exhibits reactivity toward Rat, Mouse, Human, Chicken, Frog and Hamster and has been mentioned in 11+ publications.

Quick Overview for Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 596-705) antibody (ABIN1742305)

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Reactivity: Rat, Mouse, Human, Chicken, Frog, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details

Binding Specificity: AA 596-705

Purpose: anti-RIM1

Specificity: Specific for RIM 1

Cross-Reactivity (Details): Reacts with: human (Q86UR5), rat (Q9JIR4), mouse (Q99NE5), hamster, chicken, frog. Other species not tested yet.

Purification: affinity purified

Immunogen: Recombinant protein corresponding to AA 596 to 705 from rat Rim1 (UniProt Id: Q9JIR4)

Application Details

Application Notes: WB:1 : 100 up to 1 : 1000 , IP:not tested yet ICC:1 : 200 up to 1 : 500, IHC:not tested yet IHC-P:1 : 500,

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References

Tang, Chen, Li, Metzbower, MacGillavry, Blanpied: "A trans-synaptic nanocolumn aligns neurotransmitter release to receptors." in: Nature, Vol. 536, Issue 7615, pp. 210-4, (2016) (PubMed).

Grabner, Gandini, Rehak, Le, Zamponi, Schmitz: "RIM1/2-Mediated Facilitation of Cav1.4 Channel Opening Is Required for Ca2+-Stimulated Release in Mouse Rod Photoreceptors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 38, pp. 13133-47, (2015) (PubMed).

Fernandes, Riordan, Nomura, Remmers, Kraniotis, Marshall, Kukreja, Vassar, Contractor: "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).

Ramírez-Franco, Bartolomé-Martín, Alonso, Torres, Sánchez-Prieto: "Cannabinoid type 1 receptors transiently silence glutamatergic nerve terminals of cultured cerebellar granule cells." in: PLoS ONE, Vol. 9, Issue 2, pp. e88594, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Juranek, Mukherjee, Siddiqui, Kaplan, Li, Ahnert-Hilger, Jahn, Calka: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." in: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, (2013) (PubMed).

Bello, Zanetti, Mayorga, Michaut: "RIM, Munc13, and Rab3A interplay in acrosomal exocytosis." in: Experimental cell research, Vol. 318, Issue 5, pp. 478-88, (2012) (PubMed).

Limbach, Laue, Wang, Hu, Thiede, Hultqvist, Kilimann: "Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. E392-401, (2011) (PubMed).

Leal-Ortiz, Waites, Terry-Lorenzo, Zamorano, Gundelfinger, Garner: "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 831-46, (2008) (PubMed).

Regus-Leidig, Tom Dieck, Specht, Meyer, Brandstätter: "Early steps in the assembly of photoreceptor ribbon synapses in the mouse retina: the involvement of precursor spheres." in: The Journal of comparative neurology, Vol. 512, Issue 6, pp. 814-24, (2008) (PubMed).

Jüttner, Moré, Das, Babich, Meier, Henning, Erdmann, Mu Ller, Otto, Grantyn, Rathjen: "Impaired synapse function during postnatal development in the absence of CALEB, an EGF-like protein processed by neuronal activity." in: Neuron, Vol. 46, Issue 2, pp. 233-45, (2005) (PubMed).

Target Details

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Alternative Name: RIM1

Background: Regulating synaptic membrane exocytosis protein 1, RIMS1, Rab-3-interacting molecule 1

UniProt: Q9JIR4

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity