RIMS2 antibody (AA 461-987)

Catalog No. ABIN1742308

This Rabbit Polyclonal antibody specifically detects RIMS2 in WB, IHC and ICC. It exhibits reactivity toward Rat, Mouse, Chicken, Hamster and Zebrafish (Danio rerio) and has been mentioned in 13+ publications.

Quick Overview for RIMS2 antibody (AA 461-987) (ABIN1742308)

Target: RIMS2 (Regulating Synaptic Membrane Exocytosis 2 (RIMS2))

Reactivity: Rat, Mouse, Chicken, Hamster, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIMS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-RIMS2 Antibody

Binding Specificity: AA 461-987

Specificity: RIM 2 including splice variants

Cross-Reactivity (Details): cross reactivity to RIM 1.

Purification: Affinity purified with the immunogen.

Immunogen: Recombinant protein (aa 461-987) of rat RIM 2 containing part of PDZ domain.

Application Details

Application Notes: WB: 1:100 up to 1:1000, ICC: 1:200 up to 1:500, IHC: 1:1000, IHC-P: 1:500,

Comment:

This antibody works with paraformaldehyde and methanol fixation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50µl H2O to get a 1mg/ml solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C

Storage Comment: Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.

References for anti-RIMS2 antibody (ABIN1742308)

Mendoza Schulz, Jing, Sánchez Caro, Wetzel, Dresbach, Strenzke, Wichmann, Moser: "Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse." in: The EMBO journal, Vol. 33, Issue 5, pp. 512-27, (2014) (PubMed).

de Andrade, Kunzelman, Merrill, Fuerst: "Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina." in: Molecular vision, Vol. 20, pp. 1422-33, (2014) (PubMed).

Gardezi, Li, Stanley: "Inter-channel scaffolding of presynaptic CaV2.2 via the C terminal PDZ ligand domain." in: Biology open, Vol. 2, Issue 5, pp. 492-8, (2013) (PubMed).

Limbach, Laue, Wang, Hu, Thiede, Hultqvist, Kilimann: "Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. E392-401, (2011) (PubMed).

Gebhart, Juhasz-Vedres, Zuccotti, Brandt, Engel, Trockenbacher, Kaur, Obermair, Knipper, Koschak, Striessnig: "Modulation of Cav1.3 Ca2+ channel gating by Rab3 interacting molecule." in: Molecular and cellular neurosciences, Vol. 44, Issue 3, pp. 246-59, (2010) (PubMed).

Uthaiah, Hudspeth: "Molecular anatomy of the hair cell's ribbon synapse." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12387-99, (2010) (PubMed).

Regus-Leidig, Tom Dieck, Specht, Meyer, Brandstätter: "Early steps in the assembly of photoreceptor ribbon synapses in the mouse retina: the involvement of precursor spheres." in: The Journal of comparative neurology, Vol. 512, Issue 6, pp. 814-24, (2008) (PubMed).

Kwan, Xie, Sheu, Ohtsuka, Gaisano: "Interaction between Munc13-1 and RIM is critical for glucagon-like peptide-1 mediated rescue of exocytotic defects in Munc13-1 deficient pancreatic beta-cells." in: Diabetes, Vol. 56, Issue 10, pp. 2579-88, (2007) (PubMed).

Khanna, Zougman, Stanley: "A proteomic screen for presynaptic terminal N-type calcium channel (CaV2.2) binding partners." in: Journal of biochemistry and molecular biology, Vol. 40, Issue 3, pp. 302-14, (2007) (PubMed).

Inoue, Deguchi-Tawarada, Takao-Rikitsu, Inoue, Kitajima, Ohtsuka, Takai: "ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca2+-dependent exocytosis from PC12 cells." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 6, pp. 659-72, (2006) (PubMed).

Juranek, Mukherjee, Rickmann, Martens, Calka, Südhof, Jahn: "Differential expression of active zone proteins in neuromuscular junctions suggests functional diversification." in: The European journal of neuroscience, Vol. 24, Issue 11, pp. 3043-52, (2006) (PubMed).

tom Dieck, Altrock, Kessels, Qualmann, Regus, Brauner, Fejtová, Bracko, Gundelfinger, Brandstätter: "Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex." in: The Journal of cell biology, Vol. 168, Issue 5, pp. 825-36, (2005) (PubMed).

Ohara-Imaizumi, Ohtsuka, Matsushima, Akimoto, Nishiwaki, Nakamichi, Kikuta, Nagai, Kawakami, Watanabe, Nagamatsu et al.: "ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic ..." in: Molecular biology of the cell, Vol. 16, Issue 7, pp. 3289-300, (2005) (PubMed).

Target Details for RIMS2

Target: RIMS2 (Regulating Synaptic Membrane Exocytosis 2 (RIMS2))

Alternative Name: RIM 2

Pathways: Hormone Transport, Synaptic Vesicle Exocytosis