Piccolo antibody (AA 4439-4776)
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- Target See all Piccolo (PIC) products
- Piccolo (PIC)
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Binding Specificity
- AA 4439-4776
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Reactivity
- Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Piccolo antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificity
- Specific for piccolo.
- Purification
- antiserum
- Immunogen
- Recombinant rat piccolo (aa 4439-4776).
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- Application Notes
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WB: 1 : 1000 up to 1 : 5000 (ECL detection)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 - Comment
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WB: Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed. WB: This antibody detects an additional band of ~65 kDa.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- PBS
- Handling Advice
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Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable. - Storage
- 4 °C/-20 °C
- Storage Comment
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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Neuronal MHC class I molecules are involved in excitatory synaptic transmission at the hippocampal mossy fiber synapses of marmoset monkeys." in: Cellular and molecular neurobiology, Vol. 30, Issue 6, pp. 827-39, (2010) (PubMed).
: "Rapid structural alterations of the active zone lead to sustained changes in neurotransmitter release." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 19, pp. 8836-41, (2010) (PubMed).
: "APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 46, pp. 14534-44, (2009) (PubMed).
: "Localization of the active zone proteins CAST, ELKS, and Piccolo at neuromuscular junctions." in: Neuroreport, Vol. 18, Issue 4, pp. 313-6, (2007) (PubMed).
: "Molecular dynamics of photoreceptor synapse formation in the developing chick retina." in: The Journal of comparative neurology, Vol. 506, Issue 5, pp. 822-37, (2007) (PubMed).
: "Assembly of active zone precursor vesicles: obligatory trafficking of presynaptic cytomatrix proteins Bassoon and Piccolo via a trans-Golgi compartment." in: The Journal of biological chemistry, Vol. 281, Issue 9, pp. 6038-47, (2006) (PubMed).
: "Differential expression of active zone proteins in neuromuscular junctions suggests functional diversification." in: The European journal of neuroscience, Vol. 24, Issue 11, pp. 3043-52, (2006) (PubMed).
: "Molecular profiling reveals synaptic release machinery in Merkel cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 40, pp. 14503-8, (2004) (PubMed).
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Neuronal MHC class I molecules are involved in excitatory synaptic transmission at the hippocampal mossy fiber synapses of marmoset monkeys." in: Cellular and molecular neurobiology, Vol. 30, Issue 6, pp. 827-39, (2010) (PubMed).
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- Target
- Piccolo (PIC)
- Alternative Name
- Piccolo (PIC Products)
- Background
- Synonyms: Aczonin
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