Uncoating ATPase (AA 1-650) antibody

Catalog No. ABIN1742323

This Mouse Monoclonal antibody specifically detects Uncoating ATPase in ICC, IHC (p) and WB. It exhibits reactivity toward Cow, Hamster, Human, Mouse and Rat. It has been mentioned in 12+ publications

Quick Overview for Uncoating ATPase (AA 1-650) antibody (ABIN1742323)

Target: Uncoating ATPase

Reactivity: Cow, Hamster, Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Clone: 3C5

Product Details

Binding Specificity: AA 1-650

Purpose: Mouse anti-Uncoating ATPase IgG

Specificity: Specific for uncoating ATPase.

Cross-Reactivity (Details): Reacts with: human (P11142), rat (P63018), mouse (P63017), hamster, cow. Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 1 to 650 from bovine Uncoating ATPase (UniProt Id: P19120) Epitop AA 391 to 546 from bovine Uncoating ATPase (UniProt Id: P19120)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 500 up to 1 : 5000 IHC-P:1 : 200,

Comment:

For fixation a 20 min treatment with 3.7 % PFA in PBS is recommended. When microinjected into cells, the antibody inhibits clathrin mediated endocytosis.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References

Wolf, Damme, Stroobants, DHooge, Beck, Hermans-Borgmeyer, Lüllmann-Rauch, Dierks, Lübke: "A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease." in: Disease models & mechanisms, Vol. 9, Issue 9, pp. 1015-28, (2016) (PubMed).

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

Lee, Sharma, Südhof, Shen: "Synaptic function of nicastrin in hippocampal neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 24, pp. 8973-8, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Walter, Kurps, de Wit, Schöning, Toft-Bertelsen, Lauks, Ziomkiewicz, Weiss, Schulz, Fischer von Mollard, Verhage, Sørensen: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." in: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).

García-Junco-Clemente, Cantero, Gómez-Sánchez, Linares-Clemente, Martínez-López, Luján, Fernández-Chacón: "Cysteine string protein-alpha prevents activity-dependent degeneration in GABAergic synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 21, pp. 7377-91, (2010) (PubMed).

Burré, Sharma, Tsetsenis, Buchman, Etherton, Südhof: "Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro." in: Science (New York, N.Y.), Vol. 329, Issue 5999, pp. 1663-7, (2010) (PubMed).

Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

Sharma, Burré, Südhof: "CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity." in: Nature cell biology, Vol. 13, Issue 1, pp. 30-9, (2010) (PubMed).

Newmyer, Schmid: "Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivo." in: The Journal of cell biology, Vol. 152, Issue 3, pp. 607-20, (2001) (PubMed).

Hannan, Newmyer, Schmid: "ATP- and cytosol-dependent release of adaptor proteins from clathrin-coated vesicles: A dual role for Hsc70." in: Molecular biology of the cell, Vol. 9, Issue 8, pp. 2217-29, (1998) (PubMed).

Höning, Kreimer, Robenek, Jockusch: "Receptor-mediated endocytosis is sensitive to antibodies against the uncoating ATPase (hsc70)." in: Journal of cell science, Vol. 107 ( Pt 5), pp. 1185-96, (1994) (PubMed).

Target Details

Target: Uncoating ATPase

Background:

Synonyms: hsc 70, Heat shock 70 kDa protein 8, hsc70

Antigen Information: Uncoating ATPase is involved in the removal of the clathrin coat of vesicles

UniProt: P19120