PAX6 antibody (N-Term)

Catalog No. ABIN1742329

This Mouse Monoclonal antibody specifically detects PAX6 in WB, IHC and ICC. It exhibits reactivity toward Human, Mouse, Rat and Chicken and has been mentioned in 2+ publications.

Quick Overview for PAX6 antibody (N-Term) (ABIN1742329)

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: AD2-38

Product Details anti-PAX6 Antibody

Binding Specificity: N-Term

Specificity: Specific for Pax 6.

Purification: purified IgG

Immunogen: Recombinant Pax 6. Epitope Binds to the N-terminal paired domain (PD). The epitope is present in both splice variants.

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet

Comment:

Nuclear extracts from tissues should be used for Western blot experiments to increase the concentration of Pax 6.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-PAX6 antibody (ABIN1742329)

Vasudevan, Long, Crandall, Rubenstein, Bhide: "Compartment-specific transcription factors orchestrate angiogenesis gradients in the embryonic brain." in: Nature neuroscience, Vol. 11, Issue 4, pp. 429-39, (2008) (PubMed).

Engelkamp, Rashbass, Seawright, van Heyningen: "Role of Pax6 in development of the cerebellar system." in: Development (Cambridge, England), Vol. 126, Issue 16, pp. 3585-96, (1999) (PubMed).

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: Pax 6

Background: Synonyms: Sey

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis