Homer 1/2/3 (AA 1-176), (AA 1-177), (AA 1-196) antibody

Catalog No. ABIN1742340

Cited in 2+ publications. The Rabbit Polyclonal anti-Homer 1/2/3 antibody (ABIN1742340) specifically detects Homer 1/2/3 in ICC, IHC, IHC (p) and WB. The antibody is reactive with Human, Mouse, Rat and Zebrafish (Danio rerio) samples.

Quick Overview for Homer 1/2/3 (AA 1-176), (AA 1-177), (AA 1-196) antibody (ABIN1742340)

Target: Homer 1/2/3

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Binding Specificity: AA 1-176, AA 1-177, AA 1-196

Purpose: Rabbit anti-Homer1/2/3 antibody

Specificity: The first 120 aa were used for affinity purification. According to Soloviev et al. (2000), they are homologous in Homer 1 a-d, Homer 2 a-d, and Homer 3 a-d.

Cross-Reactivity (Details): Reacts with: human (Q86YM7, Q9NSB8, Q9NSC5), rat (Q9Z214, O88801, Q9Z2X5), mouse (Q9Z2Y3, Q9QWW1, Q99JP6), zebrafish. Other species not tested yet.

Purification: affinity purified

Immunogen: Mix of recombinant proteins corresponding to AA 1 to 196 from human Homer 1, AA 1 to 176 from rat Homer 2 and AA 1 to 177 from rat Homer 3

Application Details

Application Notes: WB:1 : 1000

Comment:

ICC: PFA fixation recommended. Methanol fixation leads to a complete loss of the signal.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References

Tao-Cheng, Thein, Yang, Reese, Gallant: "Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." in: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).

Tronson, Guzman, Guedea, Huh, Gao, Schwarz, Radulovic: "Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear." in: Biological psychiatry, Vol. 68, Issue 11, pp. 1007-15, (2010) (PubMed).

Target Details

Target: Homer 1/2/3

Background:

Synonyms: VesL 1, Syn47

Antigen Information: Homer1 , 2 and 3 are post synaptic density (PSD) proteins involved in the targeting of glutamate receptors