SHANK3 antibody (C-Term)

Catalog No. ABIN1742347

This Rabbit Polyclonal antibody specifically detects SHANK3 in WB, ICC and IHC (fro). It exhibits reactivity toward Rat and Mouse and has been mentioned in 4+ publications.

Quick Overview for SHANK3 antibody (C-Term) (ABIN1742347)

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHANK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Product Details anti-SHANK3 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-Shank3 antiserum

Specificity: Specific for shank 3.

Cross-Reactivity (Details): Reacts with: rat (Q9JLU4), mouse (Q4ACU6). Other species not tested yet.

Purification: antiserum

Immunogen: Recombinant protein corresponding to residues near the carboxy terminus of rat Shank3 (UniProt Id: Q9JLU4)

Application Details

Application Notes: WB:1 : 1000

Comment:

IHC: For best results the antibody requires a mild fixation protocol (2 % PFA in sodium acetate buffer, pH 6).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References for anti-SHANK3 antibody (ABIN1742347)

Tao-Cheng, Toy, Winters, Reese, Dosemeci: "Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses." in: PLoS ONE, Vol. 11, Issue 5, pp. e0153979, (2016) (PubMed).

Wei, Ma, Ding, Jin, Liu, Chang, Hu, Yu: "Reduced Glutamate Release in Adult BTBR Mouse Model of Autism Spectrum Disorder." in: Neurochemical research, Vol. 41, Issue 11, pp. 3129-3137, (2016) (PubMed).

Wei, Ma, Liu, Ding, Jin, Wang, Hu, Yu: "Inhibition of IL-6 trans-signaling in the brain increases sociability in the BTBR mouse model of autism." in: Biochimica et biophysica acta, Vol. 1862, Issue 10, pp. 1918-25, (2016) (PubMed).

Hagmeyer, Mangus, Boeckers, Grabrucker: "Effects of trace metal profiles characteristic for autism on synapses in cultured neurons." in: Neural plasticity, Vol. 2015, pp. 985083, (2015) (PubMed).

Target Details for SHANK3

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Alternative Name: Shank3

Background:

Synonyms: SPANK 3, Shanlk-3

Antigen Information: K.O. Shanks are multidomain proteins of the postsynaptic density

UniProt: Q9JLU4

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity