GFAP antibody

Catalog No. ABIN1742363

Product Details anti-GFAP Antibody

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Chicken, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Specific for GFAP.

Purification: antiserum

Immunogen: Recombinant full length human GFAP.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 1000
IHC-P: 1 : 1000

Comment:

WB: The polyclonal antibodies are more sensitive compared to the monoclonals. ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-GFAP antibody (ABIN1742363)

Hüttenrauch, Walter, Kaufmann, Weggen, Wirths: "Limited Effects of Prolonged Environmental Enrichment on the Pathology of 5XFAD Mice." in: Molecular neurobiology, (2016) (PubMed).

Hüttenrauch, Baches, Gerth, Bayer, Weggen, Wirths: "Neprilysin deficiency alters the neuropathological and behavioral phenotype in the 5XFAD mouse model of Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 44, Issue 4, pp. 1291-302, (2015) (PubMed).

Esposito, Drexler, Braganza, Doberentz, Grote, Widman, Drosten, Eis-Hübinger, Schoch, Elger, Becker, Niehusmann: "Large-scale analysis of viral nucleic acid spectrum in temporal lobe epilepsy biopsies." in: Epilepsia, Vol. 56, Issue 2, pp. 234-43, (2015) (PubMed).

Cavanagh, Colby-Milley, Bouvier, Farso, Chabot, Quirion, Krantic: "βCTF-correlated burst of hippocampal TNFα occurs at a very early, pre-plaque stage in the TgCRND8 mouse model of Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 36, Issue 2, pp. 233-8, (2013) (PubMed).

Kaeser-Woo, Younts, Yang, Zhou, Wu, Castillo, Südhof: "Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 23, pp. 9769-80, (2013) (PubMed).

Hillmann, Hahn, Schilling, Hoffmann, Demuth, Bulic, Schneider-Axmann, Bayer, Weggen, Wirths: "No improvement after chronic ibuprofen treatment in the 5XFAD mouse model of Alzheimer's disease." in: Neurobiology of aging, Vol. 33, Issue 4, pp. 833.e39-50, (2012) (PubMed).

Ansorg, Witte, Urbach: "Age-dependent kinetics of dentate gyrus neurogenesis in the absence of cyclin D2." in: BMC neuroscience, Vol. 13, pp. 46, (2012) (PubMed).

Araya-Callís, Hiemke, Abumaria, Flugge: "Chronic psychosocial stress and citalopram modulate the expression of the glial proteins GFAP and NDRG2 in the hippocampus." in: Psychopharmacology, Vol. 224, Issue 1, pp. 209-22, (2012) (PubMed).

Kawano, Katsurabayashi, Kakazu, Yamashita, Kubo, Kubo, Okuda, Takasaki, Kubota, Mishima, Fujiwara, Harata, Iwasaki: "Long-term culture of astrocytes attenuates the readily releasable pool of synaptic vesicles." in: PLoS ONE, Vol. 7, Issue 10, pp. e48034, (2012) (PubMed).

Ribic, Flügge, Schlumbohm, Mätz-Rensing, Walter, Fuchs: "Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey." in: Behavioral and brain functions : BBF, Vol. 7, pp. 1, (2011) (PubMed).

Christensen, Schneider-Axmann, Lucassen, Bayer, Wirths: "Accumulation of intraneuronal Abeta correlates with ApoE4 genotype." in: Acta neuropathologica, Vol. 119, Issue 5, pp. 555-66, (2010) (PubMed).

Ribic, Zhang, Schlumbohm, Mätz-Rensing, Uchanska-Ziegler, Flügge, Zhang, Walter, Fuchs: "Neuronal MHC class I molecules are involved in excitatory synaptic transmission at the hippocampal mossy fiber synapses of marmoset monkeys." in: Cellular and molecular neurobiology, Vol. 30, Issue 6, pp. 827-39, (2010) (PubMed).

Wirths, Erck, Martens, Harmeier, Geumann, Jawhar, Kumar, Multhaup, Walter, Ingelsson, Degerman-Gunnarsson, Kalimo, Huitinga, Lannfelt, Bayer: "Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41517-24, (2010) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP (GFAP Products)

Synonyms: GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody