Doc 2a/b (AA 1-403) antibody

Catalog No. ABIN1742365

This Rabbit Polyclonal antibody specifically detects Doc 2a/b in ICC, IHC and WB. It exhibits reactivity toward Mouse and Rat. It has been mentioned in 2+ publications

Quick Overview for Doc 2a/b (AA 1-403) antibody (ABIN1742365)

Target: Doc 2a/b

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: AA 1-403

Purpose: Rabbit anti-Doc2a/b antibody

Specificity: Detects preferentially Doc 2a

Cross-Reactivity (Details): Reacts with: rat (P70611, P70610), mouse (Q7TNF0, P70169). No signal: zebrafish. Other species not tested yet.

Purification: affinity purified

Immunogen: Recombinant protein corresponding to AA 1 to 403 from rat Doc2a (UniProt Id: P70611)

Application Details

Application Notes: WB:1 : 1000 , IP:not tested yet ICC:1 : 100 up to 1 : 500, IHC:1 : 200, IHC-P:not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin was added for stabilization.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin was added for stabilization. Do not freeze!

References

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Schütz, Zilly, Lang, Jahn, Bruns: "A dual function for Munc-18 in exocytosis of PC12 cells." in: The European journal of neuroscience, Vol. 21, Issue 9, pp. 2419-32, (2005) (PubMed).

Target Details

Target: Doc 2a/b

Alternative Name: Doc2a/b

Background:

Synonyms: Doc2, Doc 2a, DOc 2b

Antigen Information: Doc2 a/b are binding partners of Munc 13-1

UniProt: P70611