TNC antibody (AA 23-2210)

Catalog No. ABIN1742432

The Rat Monoclonal anti-TNC antibody has been validated for WB, IHC, ICC and IHC (p). It is suitable to detect TNC in samples from Rat and Mouse. There are 5+ publications available.

Quick Overview for TNC antibody (AA 23-2210) (ABIN1742432)

Target: TNC (Tenascin C (TNC))

Reactivity: Rat, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 578

Product Details anti-TNC Antibody

Binding Specificity: AA 23-2210

Purpose: Rat anti-Tenascin-C supernatant

Specificity: Specific for tenascin-C splice variants carrying the FNIII D domain.

Cross-Reactivity (Details): Reacts with: rat, mouse (Q80YX1). Other species not tested yet.

Purification: hybridoma supernatant

Immunogen: Recombinant protein corresponding to AA 23 to 2210 from mouse Tenascin-C (UniProt Id: Q80YX1) Epitop AA 1082 to 1510 from mouse Tenascin-C (UniProt Id: Q80YX1)

Isotype: IgG2a

Application Details

Application Notes: WB:1 : 500 up to 1 : 1000 , IP:not tested yet ICC:1 : 100 up to 1 : 500, IHC:1 : 500, IHC-P:1 : 500 up to 1 : 1000,

Comment:

Tenascin-C variants detected by this antibody are downregulated during development and hardly detectable in adult animals

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References for anti-TNC antibody (ABIN1742432)

Siddiqui, Horvat-Bröcker, Faissner: "The glia-derived extracellular matrix glycoprotein tenascin-C promotes embryonic and postnatal retina axon outgrowth via the alternatively spliced fibronectin type III domain TNfnD." in: Neuron glia biology, Vol. 4, Issue 4, pp. 271-83, (2010) (PubMed).

Rigato, Garwood, Calco, Heck, Faivre-Sarrailh, Faissner: "Tenascin-C promotes neurite outgrowth of embryonic hippocampal neurons through the alternatively spliced fibronectin type III BD domains via activation of the cell adhesion molecule F3/contactin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 15, pp. 6596-609, (2002) (PubMed).

Bartsch, Bartsch, Dörries, Faissner, Weller, Ekblom, Schachner: "Expression of tenascin in the developing and adult cerebellar cortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 12, Issue 3, pp. 736-49, (1992) (PubMed).

Stern, Norris, Bronner-Fraser, Carlson, Faissner, Keynes, Schachner: "J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo." in: Development (Cambridge, England), Vol. 107, Issue 2, pp. 309-19, (1990) (PubMed).

Gatchalian, Schachner, Sanes: "Fibroblasts that proliferate near denervated synaptic sites in skeletal muscle synthesize the adhesive molecules tenascin(J1), N-CAM, fibronectin, and a heparan sulfate proteoglycan." in: The Journal of cell biology, Vol. 108, Issue 5, pp. 1873-90, (1989) (PubMed).

Target Details for TNC

Target: TNC (Tenascin C (TNC))

Alternative Name: Tenascin-C

Background:

Synonyms: TN-C, TNC, J1-200/220, Hexabrachion, Cell Adhesion Proteins

Antigen Information: Tenascin-C is a multidomain adhesion molecule

UniProt: Q80YX1

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development