GABRB3 antibody (AA 344-429)
Quick Overview for GABRB3 antibody (AA 344-429) (ABIN1742464)
Target
See all GABRB3 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 344-429
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Specificity
- Specific for GABA-A receptor beta3.
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Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
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Immunogen
- Recombinant protein (aa 344-429 in mouse) of the unprocessed precursor forced into a loop-shaped structure.
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Application Notes
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500 -
Comment
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WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
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Buffer
- PBS
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Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
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Storage
- -20 °C
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Storage Comment
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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- GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))
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Alternative Name
- GABA-A Receptor Beta3
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Background
- Synonyms: GABA(A) R beta3
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Pathways
- Sensory Perception of Sound
Target
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