GABRB3 antibody (AA 344-429)
Quick Overview for GABRB3 antibody (AA 344-429) (ABIN1742465)
Target
See all GABRB3 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 344-429
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Specificity
- Specific for GABA-A receptor beta3.
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Purification
- antiserum
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Immunogen
- Recombinant protein (aa 344-429 in mouse) of the unprocessed precursor forced into a loop-shaped structure.
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Application Notes
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500 -
Comment
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WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- PBS
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Handling Advice
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Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable. -
Storage
- 4 °C/-20 °C
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Storage Comment
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Synaptic GABAA receptor clustering without the ?2 subunit." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 31, pp. 10219-33, (2014) (PubMed).
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: "Synaptic GABAA receptor clustering without the ?2 subunit." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 31, pp. 10219-33, (2014) (PubMed).
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- GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))
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Alternative Name
- GABA-A Receptor Beta3
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Background
- Synonyms: GABA(A) R beta3
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Pathways
- Sensory Perception of Sound
Target
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