GalphaO 1/2 (AA 1-355) antibody

Catalog No. ABIN1742532

Cited in 2+ publications. The Mouse Monoclonal anti-GalphaO 1/2 antibody (Clone 101-1) (ABIN1742532) specifically detects GalphaO 1/2 in ICC, IHC, IHC (p), IP and WB. The antibody is reactive with Mouse, Rat and Zebrafish (Danio rerio) samples.

Quick Overview for GalphaO 1/2 (AA 1-355) antibody (ABIN1742532)

Target: GalphaO 1/2

Reactivity: Mouse, Rat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Grade: KO Validated

Clone: 101-1

Product Details

Binding Specificity: AA 1-355

Purpose: Mouse anti-GalphaO1/2 IgG

Specificity: Recognizes GalphaO 1 and GalphaO 2

Cross-Reactivity (Details): Reacts with: rat (P59215), mouse (P18872), zebrafish. Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 1 to 355 from rat GalphaO2 (UniProt Id: P18872)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:1 : 500 up to 1 : 1000, IHC:1 : 500 up to 1 : 2000, IHC-P:1 : 1000,

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!

References

Baron, Blex, Rohrbeck, Rachakonda, Birnbaumer, Ahnert-Hilger, Brunk: "The α-subunit of the trimeric GTPase Go2 regulates axonal growth." in: Journal of neurochemistry, Vol. 124, Issue 6, pp. 782-94, (2013) (PubMed).

Winter, Brunk, Walther, Höltje, Jiang, Peter, Takamori, Jahn, Birnbaumer, Ahnert-Hilger: "Galphao2 regulates vesicular glutamate transporter activity by changing its chloride dependence." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 18, pp. 4672-80, (2005) (PubMed).

Target Details

Target: GalphaO 1/2

Background:

Synonyms: Gnao, Gnao1, Gnao2, Go1, Go2, Go1 alpha, Go2 alpha

Antigen Information: K.O. GalphaO1 and 2 are G-proteins involved in the regulation of vesicular neurotransmitter transporters

UniProt: P18872