alpha Tubulin antibody (AA 419-435)

Catalog No. ABIN1742547

This anti-alpha Tubulin antibody is a Mouse Monoclonal antibody detecting alpha Tubulin in WB, ELISA, IP and ICC. Suitable for Human, Mouse, Rat, Saccharomyces cerevisiae, Vertebrate and Invertebrate. This Primary Antibody has been cited in 3+ publications.

Quick Overview for alpha Tubulin antibody (AA 419-435) (ABIN1742547)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae, Vertebrate, Invertebrate

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 3A2

Product Details anti-alpha Tubulin Antibody

Binding Specificity: AA 419-435

Specificity: Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide SEAREDMAALEKDYEEV (aa 419-435 in human alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 50000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC-P: 1 : 200

Comment:

ELISA: Suitable as capture antibodies for sandwich-ELISA with ABIN1742550 as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-alpha Tubulin antibody (ABIN1742547)

Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, (2015) (PubMed).

Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." in: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, (2014) (PubMed).

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, (2013) (PubMed).

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha-Tubulin

Pathways: Microtubule Dynamics