alpha Tubulin antibody (AA 443-449)

Catalog No. ABIN1742552

The Rat Monoclonal anti-alpha Tubulin antibody has been validated for WB, IHC and ICC. It is suitable to detect alpha Tubulin in samples from Chicken and Mammalian. There is 1 publication available.

Quick Overview for alpha Tubulin antibody (AA 443-449) (ABIN1742552)

Target: alpha Tubulin (TUBA1)

Reactivity: Chicken, Mammalian

Host: Rat

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 37B5

Product Details anti-alpha Tubulin Antibody

Binding Specificity: AA 443-449

Specificity: Specific for alpha-tubulin.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: 1 : 400

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-alpha Tubulin antibody (ABIN1742552)

Luchkina, Huupponen, Clarke, Coleman, Keinänen, Taira, Lauri: "Developmental switch in the kinase dependency of long-term potentiation depends on expression of GluA4 subunit-containing AMPA receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 11, pp. 4321-6, (2014) (PubMed).

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha-Tubulin

Pathways: Microtubule Dynamics