ARPC5 antibody (AA 1-151)

Catalog No. ABIN1742556

This anti-ARPC5 antibody is a Mouse Monoclonal antibody detecting ARPC5 in WB and ICC. Suitable for Human, Rat, Mouse and Hamster. This Primary Antibody has been cited in 5+ publications.

Quick Overview for ARPC5 antibody (AA 1-151) (ABIN1742556)

Target: ARPC5 (Actin Related Protein 2/3 Complex, Subunit 5, 16kDa (ARPC5))

Reactivity: Human, Rat, Mouse, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARPC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Grade: KO Validated

Clone: 323H3

Product Details anti-ARPC5 Antibody

Binding Specificity: AA 1-151

Purpose: Mouse anti-p16-Arc IgG

Specificity: Specific for p16-Arc.

Cross-Reactivity (Details): Reacts with: human (O15511), rat (Q4KLF8), mouse (Q9CPW4), hamster. Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 1 to 151 from human p16-Arc (UniProt Id: O15511) Epitop AA 19 to 24 from human p16-Arc (UniProt Id: O15511)

Isotype: IgG2a

Application Details

Application Notes: WB:1 : 100 up to 1 : 2000 , IP:not tested yet ICC:1 : 500, IHC:not tested yet IHC-P:not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-ARPC5 antibody (ABIN1742556)

Oelkers, Vinzenz, Nemethova, Jacob, Lai, Block, Szczodrak, Kerkhoff, Backert, Schlüter, Stradal, Small, Koestler, Rottner: "Microtubules as platforms for assaying actin polymerization in vivo." in: PLoS ONE, Vol. 6, Issue 5, pp. e19931, (2011) (PubMed).

Marion, Hoffmann, Holzer, Le Clainche, Martin, Sachse, Ganeva, Mangeat, Griffiths: "Ezrin promotes actin assembly at the phagosome membrane and regulates phago-lysosomal fusion." in: Traffic (Copenhagen, Denmark), Vol. 12, Issue 4, pp. 421-37, (2011) (PubMed).

Lladó, Timpson, Vilà de Muga, Moretó, Pol, Grewal, Daly, Enrich, Tebar: "Protein kinase Cdelta and calmodulin regulate epidermal growth factor receptor recycling from early endosomes through Arp2/3 complex and cortactin." in: Molecular biology of the cell, Vol. 19, Issue 1, pp. 17-29, (2008) (PubMed).

Steffen, Faix, Resch, Linkner, Wehland, Small, Rottner, Stradal: "Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes." in: Molecular biology of the cell, Vol. 17, Issue 6, pp. 2581-91, (2006) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Target Details for ARPC5

Target: ARPC5 (Actin Related Protein 2/3 Complex, Subunit 5, 16kDa (ARPC5))

Alternative Name: p16-Arc

Background:

Synonyms: Arp C5, actin polymerization complex, Arp 2/3, Arp 2/3 complex, Arp2/3 complex 16 kDa subunit , Actin-related protein 2/3 complex subunit 5

Antigen Information: K.O. p16-Arc is a member of the seven protein complex required for actin nucleation

UniProt: O15511

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization