KAP1 antibody (Internal Region)

Catalog No. ABIN1781929

This Goat Polyclonal antibody specifically detects KAP1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for KAP1 antibody (Internal Region) (ABIN1781929)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-KAP1 Antibody

Binding Specificity: Internal Region

Purpose: KAP1

Sequence: KQFNKLTEDK ADVQ

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in nuclear lysates of cell lines HeLa, Jurkat and K562 (calculated MW of 88.5 kDa according to NP_005753.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TRIM28

Background: TRIM28, tripartite motif-containing 28, FLJ29029, KAP1, RNF96, TF1B, TIF1B , KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta, tripartite motif-containing 28 protein

Molecular Weight: Expected molecular weight: 100 kDa

Gene ID: 10155

NCBI Accession: NP_005753

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus