ARNT antibody (AA 69-82)

Catalog No. ABIN1781947

This Goat Polyclonal antibody specifically detects ARNT in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for ARNT antibody (AA 69-82) (ABIN1781947)

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ARNT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ARNT Antibody

Binding Specificity: AA 69-82

Purpose: ARNT (aa69-82)

Sequence: CNDKERFARS DDEQS

Specificity: This antibody is expected to recognize all reported isoform 1 (NP_001659.1) only.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 90 kDa band observed in nuclear lysates of cell lines HeLa and HepG2 (calculated MW of 86.6 kDa according to NP_001659.1). Recommended concentration: 2-4 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Alternative Name: ARNT

Background: ARNT, aryl hydrocarbon receptor nuclear translocator, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2, OTTHUMP00000218217, OTTHUMP00000218218, OTTHUMP00000218220, class E basic helix-loop-helix protein 2, dioxin receptor, nuclear transloc

Molecular Weight: Expected molecular weight: 90 kDa

Gene ID: 405, 11863, 25242

NCBI Accession: NP_001659

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect