HNRNPA2B1 antibody (Internal Region)

Catalog No. ABIN1781995

This Goat Polyclonal antibody specifically detects HNRNPA2B1 in WB, ELISA and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for HNRNPA2B1 antibody (Internal Region) (ABIN1781995)

Target: HNRNPA2B1 (Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRNPA2B1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HNRNPA2B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-HNRNPA2B1 Antibody

Binding Specificity: Internal Region

Purpose: HNRNPA2B1

Sequence: DTIEIITDRQ S

Specificity: This antibody is expected to recognize both reported isoforms (NP_002128.1, NP_112533.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Small Intestine shows nuclear staining. Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 37 kDa band observed in lysates of cell line MCF7 and approx. 35 kDa in lysates of cell line NIH3T3 and nuclear NIH3T3 (calculated MW of 36.0 kDa according to NP_002128.1). An additional band of unknown identity was also consistently o

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HNRNPA2B1

Target: HNRNPA2B1 (Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRNPA2B1))

Alternative Name: HNRNPA2B1

Background: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1, heterogeneous nuclear ribonucleoproteins A2/B1, hnRNP A2 / hnRNP B1, nuclear ribonucleoprotein particle A2 protein

Molecular Weight: Expected molecular weight: 36 kDa

Gene ID: 3181, 53379, 362361

NCBI Accession: NP_002128, NP_112533