Fibrinogen beta Chain antibody (AA 177-190)

Catalog No. ABIN1782152

The Goat Polyclonal anti-Fibrinogen beta Chain antibody is suitable to detect Fibrinogen beta Chain in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for Fibrinogen beta Chain antibody (AA 177-190) (ABIN1782152)

Target: Fibrinogen beta Chain (FGB)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Fibrinogen beta Chain antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Fibrinogen beta Chain Antibody

Binding Specificity: AA 177-190

Purpose: fibrinogen beta chain (aa177-190)

Sequence: EKHQLYIDET VNSN

Specificity: This antibody is expected to recognize both reported isoforms (NP_005132.2, NP_001171670.1).

Cross-Reactivity: Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta and Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in Human Liver lysates (calculated MW of 55.9 kDa according to NP_005132.2). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig Liv

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Fibrinogen beta Chain

Target: Fibrinogen beta Chain (FGB)

Alternative Name: FGB

Background: FGB, fibrinogen beta chain, fibrinogen, B beta polypeptide

Molecular Weight: Expected molecular weight 60 kDa

Gene ID: 2244

NCBI Accession: NP_005132, NP_001171670