Calreticulin antibody (Internal Region)

Catalog No. ABIN1782157

This Goat Polyclonal antibody specifically detects Calreticulin in WB and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for Calreticulin antibody (Internal Region) (ABIN1782157)

Target: Calreticulin (CALR)

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Calreticulin Antibody

Binding Specificity: Internal Region

Purpose: calreticulin

Sequence: HPEIDNPEYS PDPS

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human and Rat Lung lysates (calculated MW of 48.1 kDa according to NP_004334.1). These bands correspond to earlier findings in literature with different antibodies (Conway et al, J Biol Chem. 1995 Jul 14,270(28)

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: CALR

Background: CALR, calreticulin, CRT, RO, SSA, cC1qR, CRP55, ERp60, HACBP, Sicca syndrome antigen A (autoantigen Ro, calreticulin), calregulin, endoplasmic reticulum resident protein 60, grp60

Molecular Weight: Expected molecular weight 60 kDa

Gene ID: 811, 12317, 64202

NCBI Accession: NP_004334

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response