LDHB antibody (AA 15-25)

Catalog No. ABIN1782167

The Goat Polyclonal anti-LDHB antibody has been validated for WB, IHC and ELISA. It is suitable to detect LDHB in samples from Human.

Quick Overview for LDHB antibody (AA 15-25) (ABIN1782167)

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-LDHB Antibody

Binding Specificity: AA 15-25

Purpose: LDHB (aa15-25)

Sequence: EEATVPNNKI T

Specificity: Reported variants represent identical protein: NP_002291.1, NP_001167568.1.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in Human Heart and Kidney lysates (calculated MW of 36.6 kDa according to NP_002291.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Alternative Name: LDHB

Background: LDHB, lactate dehydrogenase B, LDH-B, LDH-H, LDHBD, TRG-5, L-lactate dehydrogenase B chain, LDH heart subunit, lactate dehydrogenase H chain, renal carcinoma antigen NY-REN-46

Molecular Weight: Expected molecular weight 35 kDa

Gene ID: 3945

NCBI Accession: NP_002291

Pathways: Warburg Effect