LDHB antibody (C-Term)

Catalog No. ABIN2783283

The Rabbit Polyclonal anti-LDHB antibody (ABIN2783283) specifically detects LDHB in WB, IHC and IP. The antibody is reactive with Human, Rat, Mouse, Cow, Horse, Dog, Rabbit, Guinea Pig and Zebrafish (Danio rerio) samples.

Quick Overview for LDHB antibody (C-Term) (ABIN2783283)

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Reactivity: Human, Rat, Mouse, Cow, Horse, Dog, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-LDHB Antibody

Binding Specificity: C-Term

Purpose: LDHB Antibody - C-terminal region

Sequence: MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 93%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against LDHB. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human LDHB

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8104131

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Alternative Name: LDHB

Background:

Background Information: This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.

Gene Name: lactate dehydrogenase B

Alternative Symbols: LDH-B, LDH-H, LDHBD, TRG-5, HEL-S-281

Protein Name: L-lactate dehydrogenase B chain

Molecular Weight: 37kDa

Gene ID: 3945

NCBI Accession: NP_002291

UniProt: P07195

Pathways: Warburg Effect