SERPINB1 antibody (Internal Region)

Catalog No. ABIN1782170

The Goat Polyclonal anti-SERPINB1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect SERPINB1 in samples from Human.

Quick Overview for SERPINB1 antibody (Internal Region) (ABIN1782170)

Target: SERPINB1 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 1 (SERPINB1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SERPINB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SERPINB1 Antibody

Binding Specificity: Internal Region

Purpose: SERPINB1

Sequence: EVHSRFQSLN ADINKR

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen (neutrophils). Recommended concentration: 5 μg/mL.

Western Blot: Approx 43 kDa band observed in lysates of cell line A549 (calculated MW of 42.7 kDa according to NP_109591.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SERPINB1

Target: SERPINB1 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 1 (SERPINB1))

Alternative Name: SERPINB1

Background: SERPINB1, serpin peptidase inhibitor, clade B (ovalbumin), member 1, EI, ELANH2, LEI, M/NEI, MNEI, PI-2, PI2, leukocyte elastase inhibitor, peptidase inhibitor 2, protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived, serine (or cysteine) prot

Molecular Weight: Expected molecular weight 43 kDa

Gene ID: 1992

NCBI Accession: NP_109591