CAPG antibody (Internal Region)

Catalog No. ABIN1782185

The Goat Polyclonal anti-CAPG antibody has been validated for WB and ELISA. It is suitable to detect CAPG in samples from Human.

Quick Overview for CAPG antibody (Internal Region) (ABIN1782185)

Target: CAPG (Capping Protein (Actin Filament), Gelsolin-Like (CAPG))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CAPG antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CAPG Antibody

Binding Specificity: Internal Region

Purpose: CAPG

Sequence: NIRATERALN WDS

Specificity: This antibody is expected to recognize both reported isoforms (NP_001738.2, NP_001243069.1). Reported variants represent identical protein: NP_001243068.1, NP_001738.2.

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38 kDa band observed in lysates of cell line U937 and of Human Spleen (calculated MW of 38.5 kDa according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band runs as ~40 kDa. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CAPG

Target: CAPG (Capping Protein (Actin Filament), Gelsolin-Like (CAPG))

Alternative Name: CAPG

Background: CAPG, capping protein (actin filament), gelsolin-like, AFCP, MCP, actin regulatory protein CAP-G, actin-regulatory protein CAP-G, gelsolin-like capping protein, macrophage capping protein, macrophage-capping protein

Molecular Weight: Expected molecular weight 38 kDa

Gene ID: 822

NCBI Accession: NP_001738, NP_001243069

Pathways: Regulation of Actin Filament Polymerization