PPARD antibody (AA 39-54)

Catalog No. ABIN1803985

This Rabbit Polyclonal antibody specifically detects PPARD in WB, IHC, IHC (p) and ICC. It exhibits reactivity toward Human, Dog and Monkey.

Quick Overview for PPARD antibody (AA 39-54) (ABIN1803985)

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Reactivity: Human, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-PPARD Antibody

Binding Specificity: AA 39-54

Specificity: Human PPAR delta amino acids 39-54 (SSSYTDLSRSSSPPSL)

Purification: Immunoaffinity purified

Immunogen: Human PPAR amino acids 39-54 (SSSYTDLSRSSSPPSL). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Dog (100%), Bat, Horse (94%), Rabbit, Opossum, Turkey, Chicken (88%), Bovine, Elephant, Medaka (81%).

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: 500 μL sterile ddH20

Concentration: Lot specific

Buffer: Lyophlized, TBS, ph 7.6, 5 mg/mL BSA

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PPARD

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Alternative Name: PPARD / PPAR delta

Background: Name/Gene ID: PPARD
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: PPARD, FAAR, NUCI, PPAR-beta, PPAR-delta, NR1C2, NUC1, NUCII, Nuclear hormone receptor 1, PPARB, PPAR Delta

Gene ID: 5467

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Monocarboxylic Acid Catabolic Process, Smooth Muscle Cell Migration, Positive Regulation of fat Cell Differentiation