FeLV Gag antibody

Catalog No. ABIN180846

The Mouse Monoclonal anti-FeLV Gag antibody (Clone PF12J-10A) (ABIN180846) specifically detects FeLV Gag in EIA, WB and IHC (p). The antibody is reactive with Feline Leukemia Virus (FeLV) samples.

Quick Overview for FeLV Gag antibody (ABIN180846)

Target: FeLV Gag

Reactivity: Feline Leukemia Virus (FeLV)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PF12J-10A

Product Details

Characteristics: Synonyms: Feline Leukaemia Virus p27

Purification: Affinity chromatography on Protein A

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunohistochemistry on paraffin sections: 1: 100. Western Blot: 1: 1,500, detects a band of approximately 28 kDa under reducing conditions. Astrong band of 88 kDa representing glycosylated FeLV gag gene product may also bepresent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: PBS, pH 7.2 containing 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: FeLV Gag

Target Type: Viral Protein

Background: The oncoretrovirus FeLV is responsible for both neoplastic and non-neoplastic conditions, often resulting in a poor prognosis and fatal outcome for cats persistently infected. During persistent infection the virus, soluble viral antigens and antigen containing white blood cells are continuously present in the blood and diagnosis is mainly based upon detection of the p27 antigen - using ELISA to test for p27 as it circulates free in the bloodstream, and Immunofluorescence studies to detect p27 within the infected white cells.Synonyms: Feline Leukemia Virus