SOD1 antibody

Catalog No. ABIN180961

This Mouse Monoclonal antibody specifically detects SOD1 in IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human, Mouse and Cow.

Quick Overview for SOD1 antibody (ABIN180961)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivity: Human, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOD1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 2F5

Product Details anti-SOD1 Antibody

Specificity: This antibody recognizes Copper/Zinc Superoxide Dismutase (SOD). It does not cross react with immunoglobulins but does react weakly with Mn SOD.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Human.
Species reactivity (tested):Bovine.

Characteristics: Synonyms: SOD-1, CuZn-SOD, CuZnSOD, IPOA, ALS1, Superoxide dismutase [Cu-Zn]

Purification: Affinity Chromatography on Protein G

Immunogen: Superoxide dismutase enzyme prepared from bovine erythrocytes. Cells from immunisedmice were fused with cells of the mouse Sp-2/0-Ag14 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: This product does not require pre-treatmentof paraffin embedded sections e. g. trypsin or pronase prior to staining. This product doesnot require antigen retrieval using heat treatment methods prior to staining of paraffinsections. Recommended Positive Control: Human spleen and lymph node.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 0.1 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Alternative Name: Superoxide Dismutase 1 / SOD1

Background: Superoxide dismutases are ubiquitous metalloproteins that destroy oxygen-mediated free radicals, that are normally produced within the cells, which are toxic to biological systems. There are three forms of superoxide dismutase, including the Fe, mitochondrial (Mn) and the copper/zinc binding (Cu/Zn) form. The Cu/Zn form of SOD is utilised by most eukaryotic organisms. SOD Cu/Zn prevents oxygen-mediated free radical damage by catalysing the dismutation of the toxic superoxide radical to molecular oxygen and hydrogen peroxide. Superoxide dismutatses catalyzes the conversion of single electron reduced species of molecular oxygen to hydrogen peroxide and oxygen. Cu, Zn superoxide dismutase (SOD1) is widely distributed and comprises 90 % of total SOD. Mutations in SOD1 cause a form of familial ALS. Oxidative modifications and aggregation of SOD1 has been associated with Alzheimer's and Parkinson's disease.Synonyms: ALS1, CuZn-SOD, CuZnSOD, IPOA, SOD-1, Superoxide dismutase [Cu-Zn]

Gene ID: 6647

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis