CD25 antibody (FITC)

Catalog No. ABIN181070

The Mouse Monoclonal anti-CD25 antibody has been validated for FACS. It is suitable to detect CD25 in samples from Cow and Sheep.

Quick Overview for CD25 antibody (FITC) (ABIN181070)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Cow, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: IL-A111

Product Details anti-CD25 Antibody (FITC)

Specificity: This antibody recognises the bovine CD25 cell surface antigen, a 55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain, which is expressed by activated T cells. Cross reacts with sheep. Other species not tested.

Characteristics: Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen

Purification: Affinity chromatography on Protein G

Isotype: IgG1

Application Details

Application Notes: Flow cytometry (Neat - 1/5): use 10 μL of the suggested working dilution to label 1x10^6cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 / IL2RA

Background: The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

Gene ID: 281861

UniProt: P12342

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation