Complement C4A/C4B antibody

Catalog No. ABIN181108

This Mouse Monoclonal antibody specifically detects in WB, EIA, IHC (fro) and FACS. It exhibits reactivity toward Human.

Quick Overview for Complement C4A/C4B antibody (ABIN181108)

Target: Complement C4A/C4B

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Clone: 10-12-14

Product Details

Specificity: This antibody recognises complement component 4c (C4c).

Characteristics: Synonyms: Complement Component 4, CPAMD2, CPAMD3

Purification: Affinity chromatography on Protein A

Immunogen: Purified human C4c. Genename: C4c

Isotype: IgG1

Application Details

Application Notes: Western Blot. Flow Cytometry. ELISA: This antibody binds both C4c and C4 and so is not suitable for all ELISAapplications. Immunohistochemistry on frozen sections. Recommended Postive Control: Kidney sections from post-streptococal glomerulonephritispastients.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: Borate buffered saline, pH 8.4 containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Complement C4A/C4B

Background: The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4bs alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a-chain fragments, alpha 4 (13 kDa) and alpha 3 (25 kDa) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma.Synonyms: CPAMD2, CPAMD3, Complement Component 4

Gene ID: 720

UniProt: P0C0L4