BMP4 antibody

Catalog No. ABIN181146

The Mouse Monoclonal anti-BMP4 antibody (ABIN181146) specifically detects BMP4 in WB, IF, EIA and Func. The antibody is reactive with Human samples.

Quick Overview for BMP4 antibody (ABIN181146)

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-BMP4 Antibody

Specificity: This antibody detects BMP4.

Characteristics: Synonyms: BMP2B, BMP-2B, BMP-4, DVR4, Bone morphogenetic protein 4

Purification: Affinity Chromatography on Protein G

Immunogen: Highly pure (>98%) recombinant Human BMP-4

Isotype: IgG2b

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 2000 pg/mL of recombinant Human BMP-4 when used inconjunction with compatible secondary reagents. Western Blot: To detect Human BMP-4 by Western Blot analysis this antibody can be usedat a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human BMP-4 is 2.0-4.0 ng/lane, undernon-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhBMP-4 (5.0 ng/mL), a concentration of 0.4-0.6 μg/mL of this antibody is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for BMP4

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Alternative Name: BMP4

Background: BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix.Synonyms: BMP-2B, BMP-4, BMP2B, Bone morphogenetic protein 4, DVR4

Gene ID: 652

UniProt: P12644

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development