VEGFA antibody

Catalog No. ABIN181548

The Goat Polyclonal anti-VEGFA antibody has been validated for WB, EIA and Func. It is suitable to detect VEGFA in samples from Human.

Quick Overview for VEGFA antibody (ABIN181548)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-VEGFA Antibody

Specificity: This antibody detects VEGF.

Purification: Immunoaffinity Chromatography

Immunogen: Highly pure (> 98 %) recombinant human VEGF.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman VEGF (10 ng/mL), a concentration of 0.05-0.1 μg/mL of this antibody is required. Sandwich ELISA: To detect Human VEGF by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL is required. In conjunction with BiotinylatedAnti-Human VEGF (ABIN181546 or ABIN181545) as a Detection antibody, it allows thedetection of at least 0.2-0.4 ng/well of recombinant Human VEGF. Western Blot: To detect Human VEGF by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human VEGF is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions. Immunohistochemistry: 5.0-15.0 μg/mL with 30 minutes at RT or an overnight incubation at4 °C. This antibody stained Formalin-Fixed, Paraffin-Embedded sections of Human breastinvasive ductal carcinoma. An HRP-labeled polymer detection system was used with a DABchromogen. This antibody was also tested in an HRP-Biotin labeling detection system. Heat induced antigen retrieval with pH 6.0 Sodium Citrate buffer is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGF-A

Background: Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

Gene ID: 7422

NCBI Accession: NP_001020537

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling