Interleukin 17a antibody (Biotin)

Catalog No. ABIN181569

This anti-Interleukin 17a antibody is a Goat Polyclonal antibody detecting Interleukin 17a in WB and EIA. Suitable for Human.

Quick Overview for Interleukin 17a antibody (Biotin) (ABIN181569)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Interleukin 17a antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Interleukin 17a Antibody (Biotin)

Specificity: This antibody detects Interleukin-17A.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human IL-17A

Application Details

Application Notes: ELISA: Direct: To detect hIL-17A by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-17A. Sandwich: To detect hIL-17A by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-HumanIL-17A as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: Interleukin-17A (IL17A)

Background: IL17, also known as CTLA8, is a T cell derived hematopoietic cytokine. It was originally cloned from a T cell hybridoma produced by fusion of a mouse cytotoxic T cell clone and a rat T lymphoma. IL17 exhibits multiple biological activities on a variety of cells including: the induction of IL6, IL8 and GCSF production in fibroblasts, the enhancement of surface expression of ICAM 1 in fibroblasts, activation of NF kappaB and costimulation of T cell proliferation. IL17 is an approximately 16 kDa polypeptide of 136 amino acids. The precursor form of IL17 consists of 155 amino acids. To generate the mature IL17 (136 amino acids), the precursor cleaves a 19 amino acid signal peptide. Human IL17 shows approximately 62.5 % amino acid homology to mouse IL17 and 58 % amino acid homology to rat IL17.Synonyms: CTLA-8, CTLA8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL17

Gene ID: 3605

NCBI Accession: NP_002181

UniProt: Q16552