CD40 Ligand antibody

Catalog No. ABIN181579

This Goat Polyclonal antibody specifically detects CD40 Ligand in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for CD40 Ligand antibody (ABIN181579)

Target: CD40 Ligand (CD40LG)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-CD40 Ligand Antibody

Specificity: This antibody detects Human sCD40 Ligand / CD154.

Purification: Immunoaffinity Chromatography employing immobilized Human sCD40 Ligand matrix

Immunogen: Highly pure (> 98%) E.coli derived recombinant Human sCD40L

Application Details

Application Notes: ELISA: Indirect: To detect hsCD40-Ligand by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatiblesecondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinanthsCD40-Ligand. Sandwich: To detect hsCD40-Ligand by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL is required. In conjunction with BiotinylatedAnti-Human sCD40-Ligand as a detection antibody, it allows the detection of at least0.2 - 0.4 ng/well of recombinant hsCD40-Ligand. Western Blot: To detect hsCD40-Ligand by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hsCD40-Ligand is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for at least two weeks or (in aliquots) at -20 °C for longer.

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154 / CD40L

Background: CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5

Molecular Weight: approx. 150 kDa

Gene ID: 959

NCBI Accession: NP_000065

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints