IL-15 antibody

Catalog No. ABIN181596

The Rabbit Polyclonal anti-IL-15 antibody has been validated for WB, EIA and Func. It is suitable to detect IL-15 in samples from Mouse.

Quick Overview for IL-15 antibody (ABIN181596)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-15 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IL-15 Antibody

Specificity: This antibody detects Mouse IL-15. Other species not tested.

Purification: Immunoaffinity chromatography

Immunogen: Highly pure (> 98 %) recombinant murine IL-15.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of IL-15(10.0 ng/mL), a concentration of 0.09 - 0.149 μg/mL is required. ELISA: Indirect: To detect IL-15 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondary

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for IL-15

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Alternative Name: Interleukin-15 / IL15

Background: IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97 % and 73 % amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle. IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.Synonyms: IL-15

Gene ID: 16168

NCBI Accession: NP_032383

UniProt: P48346

Pathways: JAK-STAT Signaling, Glycosaminoglycan Metabolic Process