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CLCF1 antibody

CLCF1 Reactivity: Human WB, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN181612
  • Target See all CLCF1 Antibodies
    CLCF1 (Cardiotrophin-Like Cytokine Factor 1 (CLCF1))
    Reactivity
    • 35
    • 4
    • 2
    Human
    Host
    • 34
    • 5
    Rabbit
    Clonality
    • 36
    • 3
    Polyclonal
    Conjugate
    • 19
    • 6
    • 4
    • 3
    • 3
    • 3
    • 1
    This CLCF1 antibody is un-conjugated
    Application
    • 27
    • 18
    • 13
    • 7
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects NNT-1/BCSF-3.
    Purification
    Immunoaffinity chromatography
    Immunogen
    Highly pure (> 98 %) recombinant human NNT-1/BCSF-3
    Top Product
    Discover our top product CLCF1 Primary Antibody
  • Application Notes
    ELISA: Indirect: To detect hNNT-1/BCSF-3 by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hNNT-1/BCSF-3. Sandwich: To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human NNT-1/BCSF-3 as a detection antibody, it allows the detection ofat least 0.2 - 0.4 ng/well of recombinant hNNT-1/BCSF-3. Western Blot: To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
    Buffer
    PBS, pH 7.2
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
    Expiry Date
    6 months
  • Target
    CLCF1 (Cardiotrophin-Like Cytokine Factor 1 (CLCF1))
    Alternative Name
    CLCF1 (CLCF1 Products)
    Synonyms
    clc antibody, nr6 antibody, bsf3 antibody, nnt1 antibody, ciss2 antibody, CLCF1 antibody, BSF-3 antibody, BSF3 antibody, CISS2 antibody, CLC antibody, NNT-1 antibody, NNT1 antibody, NR6 antibody, Bsf3 antibody, Clc antibody, cardiotrophin like cytokine factor 1 antibody, cardiotrophin-like cytokine factor 1 antibody, clcf1 antibody, CLCF1 antibody, Clcf1 antibody
    Background
    NNT1 belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. IL6 family members share similarity in gene structure and have a 4 helix bundle in their protein structure. NNT1 has a sequence of 225-amino acids with a 27-aa signal peptide, a molecular mass of 22 kDa in mature form, and the highest homology to cardiotrophin-1 and ciliary neurotrophic factor. NNT1 may act as a neuroimmunoendocrine modulator of hypothalamus-pituitary-adrenal axis stress response.Synonyms: B-cell-stimulating factor 3, BSF3, CLC, Cardiotrophin-like cytokine factor 1, NNT1, Novel neurotrophin-1
    Gene ID
    23529
    NCBI Accession
    NP_037378
    UniProt
    Q9UBD9
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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