CXCL14 antibody (Biotin)

Catalog No. ABIN181661

The Rabbit Polyclonal anti-CXCL14 antibody has been validated for WB and EIA. It is suitable to detect CXCL14 in samples from Human.

Quick Overview for CXCL14 antibody (Biotin) (ABIN181661)

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL14 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CXCL14 Antibody (Biotin)

Specificity: This antibody detects BRAK.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human BRAK

Application Details

Application Notes: ELISA: Direct: To detect hBRAK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK. Sandwich: To detect hBRAK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. This In conjunction with PolyclonalAnti-Human BRAK as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/wellof recombinant hBRAK. Western blot: To detect hBRAK this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBRAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restoree in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for CXCL14

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Alternative Name: CXCL14 / BRAK

Background: BRAK/CXCL14 is a CXC chemokine constitutively expressed at the mRNA level in certain normal tissues but absent from many established tumor cell lines and human cancers. Although multiple investigators have cloned BRAK, little is known regarding the physiologic function of BRAK or the reason for decreased expression in cancer.Synonyms: C-X-C motif chemokine 14, NJAC, SCYB14, Small-inducible cytokine B14

Gene ID: 9547

NCBI Accession: NP_004878

UniProt: O95715

Pathways: Autophagy