APOE antibody (Isoform E3)

Catalog No. ABIN181668

The Rabbit Polyclonal anti-APOE antibody is suitable to detect APOE in samples from Human. It has been validated for WB and EIA.

Quick Overview for APOE antibody (Isoform E3) (ABIN181668)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-APOE Antibody

Binding Specificity: Isoform E3

Specificity: This antibody detects ApoE3. Cross reacts with ApoE2 and ApoE4.

Purification: Immunoaffinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human ApoE3

Application Details

Application Notes: ELISA: Indirect: To detect hApoE3 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3. Sandwich: To detect hApoE3 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanApoE3 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hApoE3. Western blot: To detect hApoE3 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hApoE3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: Apolipoprotein E (Apo E)

Background: Apolipoprotein E is essential for the normal catabolism of triglyceride rich lipoprotein constituents. The apolipoprotein E gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in Apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Mutations in the APOE gene confer susceptibility to Alzheimer's disease by affecting amyloid-beta deposition.Synonyms: Apo-E, ApoE

Gene ID: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism