Galectin 3 antibody

Catalog No. ABIN181684

This anti-Galectin 3 antibody is a Rabbit Polyclonal antibody detecting Galectin 3 in WB and EIA. Suitable for Human.

Quick Overview for Galectin 3 antibody (ABIN181684)

Target: Galectin 3 (LGALS3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Galectin 3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Galectin 3 Antibody

Purification: Immunoaffinity Chromatography.

Immunogen: Highly pure (> 98%) recombinant Human Galectin-3

Application Details

Application Notes: ELISAIndirect: To detect hGalectin-3 (using 100 μL/well antibody solution) a concentration of0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanGalectin-3. Sandwich: To detect Human Galectin-3 (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Polyclonal Anti-Human Galectin-3 (ABIN181681 or ABIN181682) as aDetection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinantHuman Galectin-3. Western blotTo detect Human Galectin-3, this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for Galectin 3

Target: Galectin 3 (LGALS3)

Alternative Name: Galectin-3

Target Type: Chemical

Background: Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin 3 is one of the more extensively studied members of this family and is a 30 kDa protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.Synonyms: 35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBP, Galactose-specific lectin 3, IgE-binding protein, L-31, L-34 galactoside-binding lectin, Laminin-binding protein, Lectin L-29, Lgals3, Mac-2

Gene ID: 9606

UniProt: P17931

Pathways: RTK Signaling