Thymic Stromal Lymphopoietin antibody

Catalog No. ABIN181720

The Rabbit Polyclonal anti-Thymic Stromal Lymphopoietin antibody (ABIN181720) specifically detects Thymic Stromal Lymphopoietin in WB, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for Thymic Stromal Lymphopoietin antibody (ABIN181720)

Target: Thymic Stromal Lymphopoietin (TSLP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thymic Stromal Lymphopoietin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Thymic Stromal Lymphopoietin Antibody

Specificity: This antibody detects HumanTSLP. Other species not tested.

Purification: Immunoaffinity Chromatography

Immunogen: Highly pure (> 98%) E.coli derived recombinant Human TSLP

Application Details

Application Notes: ELISA: Indirect: To detect hTSLP (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP. Sandwich: To detect hTSLP (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanTSLP as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hTSLP. Western blot: To detect hTSLP analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Thymic Stromal Lymphopoietin

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP

Background: Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.Synonyms: Thymic stroma-derived lymphopoietin, Thymic stromal lymphopoietin

Gene ID: 53603

NCBI Accession: NP_067342

UniProt: Q9JIE6