TGFA antibody (Biotin)

Catalog No. ABIN181752

The Biotin-conjugated Rabbit Polyclonal anti-TGFA antibody (ABIN181752) specifically detects TGFA in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for TGFA antibody (Biotin) (ABIN181752)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGFA antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TGFA Antibody (Biotin)

Specificity: This antibody detects TGF-α.

Purification: Affinity chromatograophy

Immunogen: Highly pure (> 98 %) recombinant human TGF-alpha

Application Details

Application Notes: ELISA: Direct: To detect hTGF-α (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTGF-α. Sandwich: To detect hTGF-α (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanTGF-α as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hTGF-α. Western blot: To detect hTGF-α this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hTGF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF-alpha (TGFA)

Background: Synonyms: EGF-like TGF, ETGF, TGF type 1, Transforming growth factor alpha

Gene ID: 7039

NCBI Accession: NP_001093161

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway