IL-13 antibody (Biotin)

Catalog No. ABIN181766

The Rabbit Polyclonal anti-IL-13 antibody has been validated for WB and EIA. It is suitable to detect IL-13 in samples from Mouse.

Quick Overview for IL-13 antibody (Biotin) (ABIN181766)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-13 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-13 Antibody (Biotin)

Specificity: This antibody detects Interleukin-13.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant murine IL-13

Application Details

Application Notes: ELISA: Direct: To detect mIL-13 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-13. Sandwich: To detect mIL-13 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-MurineIL-13 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mIL-13. Western blot: To detect mIL-13 this antibody can be used at a concentration of

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should protected from light.

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: Interleukin-13 / IL13

Background: Human IL13 was originally identified by differential screening of an anti CD28 activated human peripheral blood mononuclear cell cDNA library as an induction specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL13 (P600) cDNA as a probe. Human IL13, a pleiotropic cytokine, is produced by activated Th0, Th1 like, Th2 like, and CD8 T cells. The gene for human IL13 maps to chromosome 5 and is closely linked to the genes for IL3, IL4, IL5, and GMCSF. IL13 inhibits proinflammatory cytokine production and stimulates antibody production. It induces proliferation in the human pre myeloid cell line TF1. IL13 has multiple effects on the differentiation and functions of monocytes and macrophages. It suppresses cytotoxic functions and induces changes in the morphology of human monocytes and in the phenotype of human monocytes and B cells by upregulating MHC class II expression. IL13 will also decrease the production of nitric oxide by activated murine macrophages, leading to impaired parasiticidal activity. Human and mouse interleukin 13 share approximately 58 % amino acid sequence identity. Although human and mouse IL13 are equally active on human cells, human IL13 is much less active than mouse IL13 on mouse cells. Human IL13 and human IL4 also share approximately 30 % sequence homology and have similar biological functions.Synonyms: IL-13, NC30

Gene ID: 16163

NCBI Accession: NP_032381

UniProt: P20109

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport