N-Cadherin antibody (Cytoplasmic Domain)

Catalog No. ABIN1819143

This anti-N-Cadherin antibody is a Mouse Monoclonal antibody detecting N-Cadherin in WB, IHC, IF, IP, IHC (p) and IHC (fro). Suitable for Human and Rat.

Quick Overview for N-Cadherin antibody (Cytoplasmic Domain) (ABIN1819143)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 13A9

Product Details anti-N-Cadherin Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: Recognizes neural cadherin, otherwise known as CD325, a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry. N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells. Studies using clone 13A9 have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin see for details. Clone 13A9 has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin, see for details. Immunohistological studies have shown that clone 13A9 can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin see for details.

Purification: Protein G purified

Immunogen: Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-Fr, IHC-P, IP, WB (1:100 - 1:1000)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CDH2 / N Cadherin

Background: Name/Gene ID: CDH2
Family: Cadherin

Synonyms: CDH2, CDw325, CDHN, Cadherin-2, CD325, CD325 antigen, N Cadherin, N-cadherin, NCAD, Neural cadherin, N-cadherin 1, Neural-cadherin

Gene ID: 1000

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane