Nitrotyrosine antibody

Catalog No. ABIN1820112

This anti-Nitrotyrosine antibody is a Mouse Monoclonal antibody detecting Nitrotyrosine in WB, ELISA and IP. Suitable for .

Quick Overview for Nitrotyrosine antibody (ABIN1820112)

Target: Nitrotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 6B2-3G2

Product Details anti-Nitrotyrosine Antibody

Specificity: Recognizes both free and protein-bound nitrotyrosine (Tyr-NO2), including human serum nitro albumin, nitro insulin and nitro haemoglobin. Nitrotyrosine arises from the nitration of protein tyrosine residues, by reactive nitrogen species such as peroxynitrite and nitrogen dioxide, and can be used as a measure of NO-dependent oxidative stress and cell damage. Increased levels of nitrotyrosine are detectable in various pathological conditions including atherosclerosis, rheumatoid arthritis, cardiovascular disease, lung cancer and vasculitis. The specificity of the antibody as determined by competition ELISA is: Nitrotyrosine:95 % of inhibition, Tyrosine:0 % of inhibition, Nitrotryptophan:4 % of inhibition, Aminotyrosine:0 % of inhibition, Phosphotyrosine:2 % of inhibition, Chlorotyrosine:3 % of inhibition,.

Purification: Protein A purified

Immunogen: Protein-bound Nitrotyrosine.

Type of Immunogen: Hapten

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IP (1 μg/mL), WB (0.1 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Target Type: Chemical