NOTCH2 antibody

Catalog No. ABIN6796405

The Hamster Monoclonal anti-NOTCH2 antibody is suitable to detect NOTCH2 in samples from Mouse and Rat. It has been validated for FACS.

Quick Overview for NOTCH2 antibody (ABIN6796405)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reactivity: Mouse, Rat

Host: Hamster

Clonality: Monoclonal

Conjugate: This NOTCH2 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: HMN2-35

Product Details anti-NOTCH2 Antibody

Specificity: Recognizes Notch 2, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Signalling through Notch 2 has been implicated in the development of marginal zone B cells (MZB), the sensitization of endothelial cells to apoptosis, and the regulation of the expression of CD23 in B-cell lymphocytic leukaemia (B-CLL). Studies have also shown a correlation between a decrease in Notch 2 expression and an increase in grade of human breast cancer. Clone HMN2-35 has been shown to cross-react with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.

Purification: Protein G purified

Immunogen: Mouse Notch 2-Fc fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: NOTCH2 / NOTCH-2

Background: Name/Gene ID: NOTCH2

Synonyms: NOTCH2, AGS2, HN2, Notch (Drosophila) homolog 2, Notch homolog 2 (Drosophila), Notch 2, Notch homolog 2, HJCYS

Gene ID: 4853

UniProt: Q04721

Pathways: Notch Signaling, Stem Cell Maintenance