ATP7B antibody

Catalog No. ABIN182056

This Mouse Monoclonal antibody specifically detects ATP7B in IP, FACS and IHC (fro). It exhibits reactivity toward Cow and Pig.

Quick Overview for ATP7B antibody (ABIN182056)

Target: ATP7B (ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))

Reactivity: Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATP7B antibody is un-conjugated

Application: Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CC101

Product Details anti-ATP7B Antibody

Purification: Affinity chromatography on Protein G

Immunogen: Con A stimulated bovine lymphocytes

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation: CC101 immunoprecipitates a 215 kD molecule from bovine cells, and also recognises the swine homologue of WC1, which is a 180 kD molecule. Immunohistochemistry on frozen sections. Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for ATP7B

Target: ATP7B (ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))

Alternative Name: WC1

Background: WC1 is expressed by a population of gamma/delta TCR expression cells which lack CD2, CD4 and CD8 but express CD3. WC1 expression appears to be heterogeneous and antibodies of this cluster show differing reaction patterns. In pigs the 180kD molecule is expressed by a gamma/delta TCR positive T-cell population that is also CD2-, CD4-, CD8-.Synonyms: WC1 antigen

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process