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KARS antibody (Glu325)

This Rabbit Polyclonal antibody specifically detects KARS in WB and IP. It exhibits reactivity toward Human, Mouse and Rat.
Catalog No. ABIN1822612

Quick Overview for KARS antibody (Glu325) (ABIN1822612)

Target

See all KARS Antibodies
KARS (Lysyl-tRNA Synthetase (KARS))

Reactivity

  • 57
  • 17
  • 12
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Mouse, Rat

Host

  • 56
  • 4
Rabbit

Clonality

  • 47
  • 14
Polyclonal

Conjugate

  • 30
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This KARS antibody is un-conjugated

Application

  • 41
  • 17
  • 15
  • 14
  • 13
  • 8
  • 7
  • 7
  • 7
  • 4
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP)
  • Binding Specificity

    • 15
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    Glu325

    Specificity

    Endogenous levels of total LysRS. Species Crossreactivity: mouse, rat.

    Purification

    Affinity purified

    Immunogen

    Synthetic peptide corresponding to residues surrounding Glu325 of human LysRS.

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: IP (1:50), WB

    Usage: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4 °C with gentle shaking, overnight.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol

    Handling Advice

    Aliquot to avoid repeated freezing and thawing.

    Storage

    4 °C,-20 °C

    Storage Comment

    May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
  • Target

    KARS (Lysyl-tRNA Synthetase (KARS))

    Alternative Name

    KARS

    Background

    Name/Gene ID: KARS

    Synonyms: KARS, CMTRIB, KARS1, KARS2, KRS, Lysine--tRNA ligase, LysRS, KIAA0070, Lysine tRNA ligase, Lysyl-tRNA synthetase

    Gene ID

    3735

    UniProt

    Q15046
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