SPARC antibody (Gly53)

Catalog No. ABIN1822762

This Rabbit Polyclonal antibody specifically detects SPARC in WB, IF and IP. It exhibits reactivity toward Human, Mouse and Monkey.

Quick Overview for SPARC antibody (Gly53) (ABIN1822762)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-SPARC Antibody

Binding Specificity: Gly53

Specificity: Endogenous levels of total SPARC. Species Crossreactivity: monkey, mouse.

Purification: Affinity purified

Immunogen: Synthetic peptide corresponding to residues surrounding Gly53 of human SPARC.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:400), IP (1:50), WB

Usage: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4 °C with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: Osteonectin / SPARC

Background: Name/Gene ID: SPARC

Synonyms: SPARC, Basement-membrane protein 40, BM-40, Cysteine-rich protein, ON, Osteonectin

Gene ID: 6678

UniProt: P09486

Pathways: Autophagy